Antigenic Profile of African Horse Sickness Virus Serotype 4 VP5 and Identification of a Neutralizing Epitope Shared with Bluetongue Virus and Epizootic Hemorrhagic Disease Virus
Identifieur interne : 003965 ( Main/Exploration ); précédent : 003964; suivant : 003966Antigenic Profile of African Horse Sickness Virus Serotype 4 VP5 and Identification of a Neutralizing Epitope Shared with Bluetongue Virus and Epizootic Hemorrhagic Disease Virus
Auteurs : Jorge L. Mart Amp X0301 Nez-Torrecuadrada [Espagne] ; Jan P. M. Langeveld [Pays-Bas] ; Angel Venteo [Espagne] ; Antonio Sanz [Espagne] ; Kristian Dalsgaard [Danemark] ; William D. O. Hamilton [Royaume-Uni] ; Rob H. Meloen [Pays-Bas] ; J. Ignacio Casal [Espagne]Source :
- Virology [ 0042-6822 ] ; 1999.
English descriptors
- Teeft :
- Absorbance, Absorbance values, African horse sickness, African horse sickness virus, African horse sickness virus serotype, African horsesickness virus, African horsesickness virus serotype, Ahsv, Ahsv serotypes, Ahsv virions, Amino, Amino acid sequences, Amino acids, Amino terminus, Antibody binding, Antigenic, Antigenic determinants, Antigenic regions, Antigenic site, Antigenic sites, Antigenic structure, Antiserum, Ascitic fluid, Assay, Axis genetics, Bamhi, Bamhi fragment, Binding specificity, Biological function, Bluetongue, Bluetongue virus, Bluetongue virus serotype, Bluetongue viruses, Capsid, Capsid protein, Casal, Coli, Coli strains, Coliderived fragments, Competent cells, Competitive binding elisa, Different orbiviruses, Elisa, Epitope, Epitope mapping, Epizootic hemorrhagic disease, Equal volume, Escherichia coli, Evolutionary relationships, Expression vectors, First report, Fragment, Fusion proteins, Greater affinity, Highest serum dilution, Horse antisera, Horse rabbit, Horse sera, Horsesickness, Immune response, Immunoblotting, Immunoblotting analyses, Immunodominant region, Insect cells, Lesser extent, Linear antigenic sites, Loading buffer, Mabs, Molecular weight markers, Monoclonal antibodies, Monolayer protection assay, Mouse mabs, Negative control, Neutralization, Neutralizing, Neutralizing activity, Neutralizing antibodies, Neutralizing epitopes, Orbivirus, Orbivirus vp5s, Orbiviruses, Outer capsid proteins, Outer shell, Palyam serogroup viruses, Pepscan, Pepscan analyses, Pepscan analysis, Peptide, Phage display, Plaque reduction assay, Plasmid, Polyclonal, Polyclonal antisera, Polyclonal sera, Positive controls, Positive reaction, Preimmune rabbit serum, Primer, Rabbit, Rabbit antisera, Rabbit antiserum, Rabbit polyclonal antisera, Rabbit rabbit, Rabbit sera, Rabbit serum, Recombinant, Recombinant fragments, Recombinant plasmids, Room temperature, Serotype, Sigma, Surface exposure, Synthetic peptides, Vaccine, Vero cells, Vero monolayer protection assay, Viral infectivity, Virion, Virol, Virology, Virus, Virus neutralization, Virus serotype, Western blot analysis.
Abstract
Abstract: African horse sickness virus (AHSV) causes a fatal disease in horses. The virus capsid is composed of a double protein layer, the outermost of which is formed by two proteins: VP2 and VP5. VP2 is known to determine the serotype of the virus and to contain the neutralizing epitopes. The biological function of VP5, the other component of the capsid, is unknown. In this report, AHSV VP5, expressed in insect cells alone or together with VP2, was able to induce AHSV-specific neutralizing antibodies. Moreover, two VP5-specific monoclonal antibodies (MAbs) that were able to neutralize the virus in a plaque reduction assay were generated. To dissect the antigenic structure of AHSV VP5, the protein was cloned inEscherichia coliusing the pET3 system. The immunoreactivity of both MAbs, and horse and rabbit polyclonal antisera, with 17 overlapping fragments from VP5 was analyzed. The most immunodominant region was found in the N-terminal 330 residues of VP5, defining two antigenic regions, I (residues 151–200) and II (residues 83–120). The epitopes were further defined by PEPSCAN analysis with 12mer peptides, which determined eight antigenic sites in the N-terminal half of the molecule. Neutralizing epitopes were defined at positions 85–92 (PDPLSPGE) for MAb 10AE12 and at 179–185 (EEDLRTR) for MAb 10AC6. Epitope 10AE12 is highly conserved between the different orbiviruses. MAb 10AE12 was able to recognize bluetongue virus VP5 and epizootic hemorrhagic disease virus VP5 by several techniques. These data will be especially useful for vaccine development and diagnostic purposes.
Url:
DOI: 10.1006/viro.1999.9680
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002091
- to stream Istex, to step Curation: 002091
- to stream Istex, to step Checkpoint: 001218
- to stream Main, to step Merge: 003A10
- to stream Main, to step Curation: 003965
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antigenic Profile of African Horse Sickness Virus Serotype 4 VP5 and Identification of a Neutralizing Epitope Shared with Bluetongue Virus and Epizootic Hemorrhagic Disease Virus</title><author><name sortKey="Mart Amp X0301 Nez Torrecuadrada, Jorge L" sort="Mart Amp X0301 Nez Torrecuadrada, Jorge L" uniqKey="Mart Amp X0301 Nez Torrecuadrada J" first="Jorge L." last="Mart Amp X0301 Nez-Torrecuadrada">Jorge L. Mart Amp X0301 Nez-Torrecuadrada</name></author><author><name sortKey="Langeveld, Jan P M" sort="Langeveld, Jan P M" uniqKey="Langeveld J" first="Jan P. M." last="Langeveld">Jan P. M. Langeveld</name></author><author><name sortKey="Venteo, Angel" sort="Venteo, Angel" uniqKey="Venteo A" first="Angel" last="Venteo">Angel Venteo</name></author><author><name sortKey="Sanz, Antonio" sort="Sanz, Antonio" uniqKey="Sanz A" first="Antonio" last="Sanz">Antonio Sanz</name></author><author><name sortKey="Dalsgaard, Kristian" sort="Dalsgaard, Kristian" uniqKey="Dalsgaard K" first="Kristian" last="Dalsgaard">Kristian Dalsgaard</name></author><author><name sortKey="Hamilton, William D O" sort="Hamilton, William D O" uniqKey="Hamilton W" first="William D. O." last="Hamilton">William D. O. Hamilton</name></author><author><name sortKey="Meloen, Rob H" sort="Meloen, Rob H" uniqKey="Meloen R" first="Rob H." last="Meloen">Rob H. Meloen</name></author><author><name sortKey="Casal, J Ignacio" sort="Casal, J Ignacio" uniqKey="Casal J" first="J. Ignacio" last="Casal">J. Ignacio Casal</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E7CC67E81A948963BECCAA03651F0BD335E0AE6C</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1006/viro.1999.9680</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-0F7RXTC6-M/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002091</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002091</idno><idno type="wicri:Area/Istex/Curation">002091</idno><idno type="wicri:Area/Istex/Checkpoint">001218</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001218</idno><idno type="wicri:doubleKey">0042-6822:1999:Mart Amp X0301 Nez Torrecuadrada J:antigenic:profile:of</idno><idno type="wicri:Area/Main/Merge">003A10</idno><idno type="wicri:Area/Main/Curation">003965</idno><idno type="wicri:Area/Main/Exploration">003965</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Antigenic Profile of African Horse Sickness Virus Serotype 4 VP5 and Identification of a Neutralizing Epitope Shared with Bluetongue Virus and Epizootic Hemorrhagic Disease Virus</title><author><name sortKey="Mart Amp X0301 Nez Torrecuadrada, Jorge L" sort="Mart Amp X0301 Nez Torrecuadrada, Jorge L" uniqKey="Mart Amp X0301 Nez Torrecuadrada J" first="Jorge L." last="Mart Amp X0301 Nez-Torrecuadrada">Jorge L. Mart Amp X0301 Nez-Torrecuadrada</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Inmunologı́a y Genética Aplicada SA (INGENASA), Hnos Garcia Noblejas 41, 28037, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Langeveld, Jan P M" sort="Langeveld, Jan P M" uniqKey="Langeveld J" first="Jan P. M." last="Langeveld">Jan P. M. Langeveld</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>ID-DLO, P.O. Box 65, NL-8200, Lelystad</wicri:regionArea><wicri:noRegion>Lelystad</wicri:noRegion></affiliation></author><author><name sortKey="Venteo, Angel" sort="Venteo, Angel" uniqKey="Venteo A" first="Angel" last="Venteo">Angel Venteo</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Inmunologı́a y Genética Aplicada SA (INGENASA), Hnos Garcia Noblejas 41, 28037, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Sanz, Antonio" sort="Sanz, Antonio" uniqKey="Sanz A" first="Antonio" last="Sanz">Antonio Sanz</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Inmunologı́a y Genética Aplicada SA (INGENASA), Hnos Garcia Noblejas 41, 28037, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Dalsgaard, Kristian" sort="Dalsgaard, Kristian" uniqKey="Dalsgaard K" first="Kristian" last="Dalsgaard">Kristian Dalsgaard</name><affiliation wicri:level="1"><country xml:lang="fr">Danemark</country><wicri:regionArea>Danish Veterinary Institute for Virus Research, DK-4771, Kalvehave</wicri:regionArea><wicri:noRegion>Kalvehave</wicri:noRegion></affiliation></author><author><name sortKey="Hamilton, William D O" sort="Hamilton, William D O" uniqKey="Hamilton W" first="William D. O." last="Hamilton">William D. O. Hamilton</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Axis Genetics plc. Brabaham, Cambridge, CB2 4AZ</wicri:regionArea><wicri:noRegion>CB2 4AZ</wicri:noRegion></affiliation></author><author><name sortKey="Meloen, Rob H" sort="Meloen, Rob H" uniqKey="Meloen R" first="Rob H." last="Meloen">Rob H. Meloen</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>ID-DLO, P.O. Box 65, NL-8200, Lelystad</wicri:regionArea><wicri:noRegion>Lelystad</wicri:noRegion></affiliation></author><author><name sortKey="Casal, J Ignacio" sort="Casal, J Ignacio" uniqKey="Casal J" first="J. Ignacio" last="Casal">J. Ignacio Casal</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Inmunologı́a y Genética Aplicada SA (INGENASA), Hnos Garcia Noblejas 41, 28037, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">257</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="449">449</biblScope><biblScope unit="page" to="459">459</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Absorbance</term><term>Absorbance values</term><term>African horse sickness</term><term>African horse sickness virus</term><term>African horse sickness virus serotype</term><term>African horsesickness virus</term><term>African horsesickness virus serotype</term><term>Ahsv</term><term>Ahsv serotypes</term><term>Ahsv virions</term><term>Amino</term><term>Amino acid sequences</term><term>Amino acids</term><term>Amino terminus</term><term>Antibody binding</term><term>Antigenic</term><term>Antigenic determinants</term><term>Antigenic regions</term><term>Antigenic site</term><term>Antigenic sites</term><term>Antigenic structure</term><term>Antiserum</term><term>Ascitic fluid</term><term>Assay</term><term>Axis genetics</term><term>Bamhi</term><term>Bamhi fragment</term><term>Binding specificity</term><term>Biological function</term><term>Bluetongue</term><term>Bluetongue virus</term><term>Bluetongue virus serotype</term><term>Bluetongue viruses</term><term>Capsid</term><term>Capsid protein</term><term>Casal</term><term>Coli</term><term>Coli strains</term><term>Coliderived fragments</term><term>Competent cells</term><term>Competitive binding elisa</term><term>Different orbiviruses</term><term>Elisa</term><term>Epitope</term><term>Epitope mapping</term><term>Epizootic hemorrhagic disease</term><term>Equal volume</term><term>Escherichia coli</term><term>Evolutionary relationships</term><term>Expression vectors</term><term>First report</term><term>Fragment</term><term>Fusion proteins</term><term>Greater affinity</term><term>Highest serum dilution</term><term>Horse antisera</term><term>Horse rabbit</term><term>Horse sera</term><term>Horsesickness</term><term>Immune response</term><term>Immunoblotting</term><term>Immunoblotting analyses</term><term>Immunodominant region</term><term>Insect cells</term><term>Lesser extent</term><term>Linear antigenic sites</term><term>Loading buffer</term><term>Mabs</term><term>Molecular weight markers</term><term>Monoclonal antibodies</term><term>Monolayer protection assay</term><term>Mouse mabs</term><term>Negative control</term><term>Neutralization</term><term>Neutralizing</term><term>Neutralizing activity</term><term>Neutralizing antibodies</term><term>Neutralizing epitopes</term><term>Orbivirus</term><term>Orbivirus vp5s</term><term>Orbiviruses</term><term>Outer capsid proteins</term><term>Outer shell</term><term>Palyam serogroup viruses</term><term>Pepscan</term><term>Pepscan analyses</term><term>Pepscan analysis</term><term>Peptide</term><term>Phage display</term><term>Plaque reduction assay</term><term>Plasmid</term><term>Polyclonal</term><term>Polyclonal antisera</term><term>Polyclonal sera</term><term>Positive controls</term><term>Positive reaction</term><term>Preimmune rabbit serum</term><term>Primer</term><term>Rabbit</term><term>Rabbit antisera</term><term>Rabbit antiserum</term><term>Rabbit polyclonal antisera</term><term>Rabbit rabbit</term><term>Rabbit sera</term><term>Rabbit serum</term><term>Recombinant</term><term>Recombinant fragments</term><term>Recombinant plasmids</term><term>Room temperature</term><term>Serotype</term><term>Sigma</term><term>Surface exposure</term><term>Synthetic peptides</term><term>Vaccine</term><term>Vero cells</term><term>Vero monolayer protection assay</term><term>Viral infectivity</term><term>Virion</term><term>Virol</term><term>Virology</term><term>Virus</term><term>Virus neutralization</term><term>Virus serotype</term><term>Western blot analysis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: African horse sickness virus (AHSV) causes a fatal disease in horses. The virus capsid is composed of a double protein layer, the outermost of which is formed by two proteins: VP2 and VP5. VP2 is known to determine the serotype of the virus and to contain the neutralizing epitopes. The biological function of VP5, the other component of the capsid, is unknown. In this report, AHSV VP5, expressed in insect cells alone or together with VP2, was able to induce AHSV-specific neutralizing antibodies. Moreover, two VP5-specific monoclonal antibodies (MAbs) that were able to neutralize the virus in a plaque reduction assay were generated. To dissect the antigenic structure of AHSV VP5, the protein was cloned inEscherichia coliusing the pET3 system. The immunoreactivity of both MAbs, and horse and rabbit polyclonal antisera, with 17 overlapping fragments from VP5 was analyzed. The most immunodominant region was found in the N-terminal 330 residues of VP5, defining two antigenic regions, I (residues 151–200) and II (residues 83–120). The epitopes were further defined by PEPSCAN analysis with 12mer peptides, which determined eight antigenic sites in the N-terminal half of the molecule. Neutralizing epitopes were defined at positions 85–92 (PDPLSPGE) for MAb 10AE12 and at 179–185 (EEDLRTR) for MAb 10AC6. Epitope 10AE12 is highly conserved between the different orbiviruses. MAb 10AE12 was able to recognize bluetongue virus VP5 and epizootic hemorrhagic disease virus VP5 by several techniques. These data will be especially useful for vaccine development and diagnostic purposes.</div></front></TEI><affiliations><list><country><li>Danemark</li><li>Espagne</li><li>Pays-Bas</li><li>Royaume-Uni</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Mart Amp X0301 Nez Torrecuadrada, Jorge L" sort="Mart Amp X0301 Nez Torrecuadrada, Jorge L" uniqKey="Mart Amp X0301 Nez Torrecuadrada J" first="Jorge L." last="Mart Amp X0301 Nez-Torrecuadrada">Jorge L. Mart Amp X0301 Nez-Torrecuadrada</name></region><name sortKey="Casal, J Ignacio" sort="Casal, J Ignacio" uniqKey="Casal J" first="J. Ignacio" last="Casal">J. Ignacio Casal</name><name sortKey="Sanz, Antonio" sort="Sanz, Antonio" uniqKey="Sanz A" first="Antonio" last="Sanz">Antonio Sanz</name><name sortKey="Venteo, Angel" sort="Venteo, Angel" uniqKey="Venteo A" first="Angel" last="Venteo">Angel Venteo</name></country><country name="Pays-Bas"><noRegion><name sortKey="Langeveld, Jan P M" sort="Langeveld, Jan P M" uniqKey="Langeveld J" first="Jan P. M." last="Langeveld">Jan P. M. Langeveld</name></noRegion><name sortKey="Meloen, Rob H" sort="Meloen, Rob H" uniqKey="Meloen R" first="Rob H." last="Meloen">Rob H. Meloen</name></country><country name="Danemark"><noRegion><name sortKey="Dalsgaard, Kristian" sort="Dalsgaard, Kristian" uniqKey="Dalsgaard K" first="Kristian" last="Dalsgaard">Kristian Dalsgaard</name></noRegion></country><country name="Royaume-Uni"><noRegion><name sortKey="Hamilton, William D O" sort="Hamilton, William D O" uniqKey="Hamilton W" first="William D. O." last="Hamilton">William D. O. Hamilton</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003965 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003965 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:E7CC67E81A948963BECCAA03651F0BD335E0AE6C
|texte= Antigenic Profile of African Horse Sickness Virus Serotype 4 VP5 and Identification of a Neutralizing Epitope Shared with Bluetongue Virus and Epizootic Hemorrhagic Disease Virus
}}
| This area was generated with Dilib version V0.6.33. |  |